Photoelectrochemical competitive DNA hybridization assay using semiconductor quantum dot conjugated oligonucleotides.
Identifieur interne : 001215 ( Main/Exploration ); précédent : 001214; suivant : 001216Photoelectrochemical competitive DNA hybridization assay using semiconductor quantum dot conjugated oligonucleotides.
Auteurs : RBID : pubmed:21442370English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : DNA, DNA, Single-Stranded, Oligonucleotides.
- methods : Electrochemical Techniques, Nucleic Acid Hybridization.
- Photochemistry, Quantum Dots, Semiconductors, Sensitivity and Specificity.
Abstract
A competitive DNA hybridization assay based on the photoelectrochemistry of the semiconductor quantum dot-single stranded DNA conjugates (QD-ssDNA) was developed. Hybridization of QD-ssDNA with the capture probe DNA immobilized on the indium-tin oxide electrodes enables photocurrent generation when the electrochemical cell was illuminated with a light source. Upon the competition between QD-ssDNA and single-stranded target DNA, the photocurrent response decreased with the increase in the target DNA concentration. A linear relationship between the photocurrent and the target DNA concentration was obtained (R(2) = 0.991). The selectivity of system towards the target DNA was also demonstrated using non-complementary sample.
DOI: 10.1007/s00216-011-4827-4
PubMed: 21442370
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Photoelectrochemical competitive DNA hybridization assay using semiconductor quantum dot conjugated oligonucleotides.</title><author><name sortKey="Ba, Deniz" uniqKey="Ba D">Deniz Baş</name><affiliation wicri:level="1"><nlm:affiliation>Department of Food Engineering, Hacettepe University, Beytepe, Ankara, Turkey.</nlm:affiliation><country xml:lang="fr">Turquie</country><wicri:regionArea>Department of Food Engineering, Hacettepe University, Beytepe, Ankara</wicri:regionArea></affiliation></author><author><name sortKey="Boyaci, Ismail Hakki" uniqKey="Boyaci I">Ismail Hakki Boyaci</name></author></titleStmt><publicationStmt><date when="2011">2011</date><idno type="doi">10.1007/s00216-011-4827-4</idno><idno type="RBID">pubmed:21442370</idno><idno type="pmid">21442370</idno><idno type="wicri:Area/Main/Corpus">001479</idno><idno type="wicri:Area/Main/Curation">001479</idno><idno type="wicri:Area/Main/Exploration">001215</idno></publicationStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>DNA (chemistry)</term><term>DNA, Single-Stranded (chemistry)</term><term>Electrochemical Techniques (methods)</term><term>Nucleic Acid Hybridization (methods)</term><term>Oligonucleotides (chemistry)</term><term>Photochemistry</term><term>Quantum Dots</term><term>Semiconductors</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA</term><term>DNA, Single-Stranded</term><term>Oligonucleotides</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Electrochemical Techniques</term><term>Nucleic Acid Hybridization</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Photochemistry</term><term>Quantum Dots</term><term>Semiconductors</term><term>Sensitivity and Specificity</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A competitive DNA hybridization assay based on the photoelectrochemistry of the semiconductor quantum dot-single stranded DNA conjugates (QD-ssDNA) was developed. Hybridization of QD-ssDNA with the capture probe DNA immobilized on the indium-tin oxide electrodes enables photocurrent generation when the electrochemical cell was illuminated with a light source. Upon the competition between QD-ssDNA and single-stranded target DNA, the photocurrent response decreased with the increase in the target DNA concentration. A linear relationship between the photocurrent and the target DNA concentration was obtained (R(2) = 0.991). The selectivity of system towards the target DNA was also demonstrated using non-complementary sample.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="MEDLINE"><PMID Version="1">21442370</PMID><DateCreated><Year>2011</Year><Month>04</Month><Day>11</Day></DateCreated><DateCompleted><Year>2011</Year><Month>08</Month><Day>04</Day></DateCompleted><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1618-2650</ISSN><JournalIssue CitedMedium="Internet"><Volume>400</Volume><Issue>3</Issue><PubDate><Year>2011</Year><Month>May</Month></PubDate></JournalIssue><Title>Analytical and bioanalytical chemistry</Title><ISOAbbreviation>Anal Bioanal Chem</ISOAbbreviation></Journal><ArticleTitle>Photoelectrochemical competitive DNA hybridization assay using semiconductor quantum dot conjugated oligonucleotides.</ArticleTitle><Pagination><MedlinePgn>703-7</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00216-011-4827-4</ELocationID><Abstract><AbstractText>A competitive DNA hybridization assay based on the photoelectrochemistry of the semiconductor quantum dot-single stranded DNA conjugates (QD-ssDNA) was developed. Hybridization of QD-ssDNA with the capture probe DNA immobilized on the indium-tin oxide electrodes enables photocurrent generation when the electrochemical cell was illuminated with a light source. Upon the competition between QD-ssDNA and single-stranded target DNA, the photocurrent response decreased with the increase in the target DNA concentration. A linear relationship between the photocurrent and the target DNA concentration was obtained (R(2) = 0.991). The selectivity of system towards the target DNA was also demonstrated using non-complementary sample.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Baş</LastName><ForeName>Deniz</ForeName><Initials>D</Initials><Affiliation>Department of Food Engineering, Hacettepe University, Beytepe, Ankara, Turkey.</Affiliation></Author><Author ValidYN="Y"><LastName>Boyaci</LastName><ForeName>Ismail Hakki</ForeName><Initials>IH</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType>Evaluation Studies</PublicationType><PublicationType>Journal Article</PublicationType><PublicationType>Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2011</Year><Month>03</Month><Day>26</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Anal Bioanal Chem</MedlineTA><NlmUniqueID>101134327</NlmUniqueID></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>DNA, Single-Stranded</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Oligonucleotides</NameOfSubstance></Chemical><Chemical><RegistryNumber>9007-49-2</RegistryNumber><NameOfSubstance>DNA</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName MajorTopicYN="N">DNA</DescriptorName><QualifierName MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">DNA, Single-Stranded</DescriptorName><QualifierName MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Electrochemical Techniques</DescriptorName><QualifierName MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Nucleic Acid Hybridization</DescriptorName><QualifierName MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Oligonucleotides</DescriptorName><QualifierName MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Photochemistry</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="Y">Quantum Dots</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="Y">Semiconductors</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Sensitivity and Specificity</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2010</Year><Month>11</Month><Day>12</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2011</Year><Month>2</Month><Day>19</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2011</Year><Month>2</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="aheadofprint"><Year>2011</Year><Month>3</Month><Day>26</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2011</Year><Month>3</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2011</Year><Month>3</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2011</Year><Month>8</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="doi">10.1007/s00216-011-4827-4</ArticleId><ArticleId IdType="pubmed">21442370</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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